Base Thermal Cycler

DNA Engine or Dyad Disciple cycler

Sample capacity

96-well PCR plate or 96 x 0.2 ml tubes

Sample volume

10–100 µl (20 µl recommended)

Dimensions (W x D x H)

23 x 23 x 18 cm (excluding thermal cycler)

Weight

6.8 kg (excluding thermal cycler)

Fluorescence excitation range

Channel 1: 450–490 nm
Channel 2: 500–535 nm
Channel 3: 555–585 nm
Channel 4: 620–650 nm

Fluorescence detection range

Channel 1: 515–530 nm
Channel 2: 560–580 nm
Channel 3: 610–650 nm
Channel 4: 675–730 nm

Linear dynamic range of starting copy number

Up to 10 orders of magnitude

Detection limit of template starting copy number

Down to single initial template copy detection

Gradient specifications

Gradient range

30–105°C

Temperature differential range

1°C–24°C

Gradient accuracy

±0.3°C of programmed target at end columns within 30 sec after gradient step timer starts, NIST traceable

Temperature uniformity

±0.4°C, in column, well-to-well, within 30 sec of reaching target temperature

Gradient calculator accuracy

±0.4°C of actual well temperature

Note: Specifications subject to change without notice.

Minimum Computer Specifications

Operating system

Windows XP Professional

Processor speed

1.5 GHz

Storage capacity

20 GB hard drive

Communications port

USB 2.0 high-speed

Display

1,024 x 768 pixel minimum screen resolution, 256 colors

Memory

512 MB RAM

Note: Specifications subject to change without notice.