Description

The ECM 399 is an electroporation system specifically designed to produce the precise field strengths and pulse lengths required for the transfection of mammalian and transformation of bacterial and yeast cells. The ECM 399 utilizes the new BTX Power Platform Technology design and novel digital user interface. The single-dial encoder simplifies the operation of ECM 399. Portable, convenient, and user-friendly are major features of this unit.

 

Applications

Bacteria/Yeast Transformation
In the High Voltage (HV) Mode, the ECM 399 is optimized to electroporate E. coli and other bacteria and yeast for construction of cDNA libraries or mutagenesis.

 

Mammalian Cell Transfection
In the Low Voltage (LV) Mode, the ECM 399 is designed to deliver a pulse length of approximately 26 msec using the 4mm gap cuvette, at a volume of 400µl using conductive media such as PBS. This protocol has been found to be ideal for transfection of lymphoid, myeloid, glial, fibroblast and Cos 7 cells.1 Protocols for these and other mammalian cells can be optimized by varying the voltage and/or the pulse length. Adjusting the voltage adjustment knob varies the voltage, while the pulse length may be changed by varying the sample volume or buffer composition.

 

Technical Specifications